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	Provedor de dados BJMBR (2) Autor Anziliero,D. (2) Flores,E.F. (2) Weiblen,R. (2) Brum,M.C.S. (1) Kreutz,L.C. (1) Spilki,F. (1) Weiss,M. (1) Mais... Palavra-chave Cattle pathogen (1) Control (1) Cytokine (1) Differential vaccine (1) Immunization (1) Immunostimulant (1) Innate immune response (1) MRNA (1) ORF virus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2014 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain BJMBR Weiss,M.; Brum,M.C.S.; Anziliero,D.; Weiblen,R.; Flores,E.F.. A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones were obtained out of approximately 5000 viral plaques. Deletion of the gE gene and the presence of the GFP marker in the genome of recombinant viruses were confirmed by PCR. Despite forming smaller plaques, the BoHV-1△gE recombinants replicated in MDBK cells with similar kinetics and to similar titers to that of the parental virus (SV56/90), demonstrating that the gE deletion had no deleterious effects... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle pathogen; Immunization; Control; Differential vaccine. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900843 Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice BJMBR Anziliero,D.; Weiblen,R.; Kreutz,L.C.; Spilki,F.; Flores,E.F.. The immunostimulatory properties of inactivated Parapoxvirus ovis (iPPVO) have long been investigated in different animal species and experimental settings. In this study, we investigated the effects of iPPVO on cytokine expression in mice after intraperitoneal inoculation. Spleen and sera collected from iPPVO-treated mice at intervals after inoculation were submitted to cytokine mRNA determination by real-time PCR (qPCR), serum protein concentration by ELISA, and interferon (IFN)-α/β activity by bioassay. The spleen of iPPVO-treated animals showed a significant increase in mRNA expression of all cytokines assayed, with different kinetics and magnitude. Proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α), and IL-8 mRNA peaked... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytokine; MRNA; ORF virus; Immunostimulant; Innate immune response. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200110 Registros recuperados: 2 Primeira ... 1 ... Última

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